Hi @bk-user,
Probably the easiest way is to just call the regedit command line with Run Process
If you saved that reg file as AutoSelectCertificateForUrls.reg, then something like this
Run Process regedit.exe /s AutoSelectCertificateForUrls.reg
This may work as long as your user has sufficient rights to run regedit, otherwise you could explore winreg python module.
Hope that helps,
Dave.